Journal:

Article Title: The lobster mandibular organ produces soluble and membrane-bound forms of 3-hydroxy-3-methylglutaryl-CoA reductase

doi: 10.1042/BJ20031930

Figure Lengend Snippet: Effects of phosphatase and ATP on HMGR activity Tissue and recombinant protein samples with HMGR activity (5–10 μg of protein in each sample) were incubated for 30 min alone in HMGR buffer without KH 2 PO 4 (control), with 1 unit of lambda protein phosphatase (Phosphatase), with 1×10 −8 M ATP (ATP) or with 1×10 −8 M ATP plus approx. 100 μg of rat liver whole homogenate (ATP+rat liver homogenate). For both treatments containing ATP, EDTA was removed from the HMGR buffer. HMGR activity [nmol·(mg of protein) −1 ·min −1 , ±S.E.M., n =4 determinations] was measured after treatment. N.D., not determined.

Article Snippet: Rec-HMGR1 (5 μg of protein) or MO fractions (10 μg of protein) in 50 μl of HMGR buffer without KH 2 PO 4 were incubated alone (Control), with lambda protein phosphatase (1, 3 and 10 units; New England Biolabs) with ATP (1×10 −8 to 10 −5 M; Sigma) or with 1×10 −8 M ATP and approx.

Techniques: Activity Assay, Recombinant, Incubation, Purification